Figure 7.

Characterization of K7 KO cells. (A) Northern blot of RNA isolated from the AX2 wild type and the two K7 mutants (KO1 and KO2) at T12 was hybridized to a K7 probe. After prolonged exposure, the RNA from the KO cells shows only background hybridization to the ribosomal RNA (R). (B) Southern blot of EcoRV-restricted genomic DNA isolated from the same strains was hybridized to the K7 probe. The band hybridizing to the K7 probe shows a 1-kb increase in size, which is expected from the gene replacement event. The lack of hybridization to additional bands indicates that additional copies of the K7 gene disruption construct did not integrate elsewhere in the genome.