Table 1.
Primer name | Sequence |
Eco OMP | GAATTCATGGCAGAGGACGGGCCACAGAAG |
OMP Xho | CTCGAGTCAGAGCTGGTTAAACACCACAG |
AC3F | CAGCCAGCAGCCAACATGCCG |
AC3R | CTTCCAGCTCATCCTGCTGCTG |
GolfF2 | ATGGGCCTATGCTACAGCCTGCGG |
GolfR2 | CTGACCGGAACTGGTTCTCAGGGTTG |
CNGF2 | GGTGCTGGATTACTTCTCAGACAC |
CNGR | CCAGGTAGCCATATTCAGGGTCAG |
GAPDH-F | ATGGTGAAGGTCGGTGTGAACG |
GAPDH-R | AGTGATGGCATGGACTGTGGTC |
M72-F | GTTAACATGGCTGCAGAGAATCAATC |
M72-R | GAATTCAAAGACTCTTCTCCTCAGTG |
P2-F | GGATCCATGACCTGGGGAAACTGGAC |
P2-R | GAATTCTAGTTTCTGAGGGCCCAGAG |
I7-F | GTTAACATGGAGCGAAGGAACCACAC |
I7-R | GAATTCACCATCTCTGCTGGATTTC |
OR118F | ATGGCGAACAGCACTACTGTTACTG |
OR118R | TGTCTGGCTGAACTTTTGGAACTTGC |
OR203F | ATGGAGGTGAACAGGACCCTGGTGACT |
OR203R | TGGCTTCATGATTTTTCTCAGAGCC |
GFP-LTF | GAATTCGCCACCATGGTGAGCAAGGGCG |
GFP-LTR | CAATTGCTTAAGATACATTGATGAGTTTGG |
GFP-MH2 | AATGAATGCAATAGTTGTTGATAACTTGTTTATTG |
GFP-MH2R | CAATAAACAAGTTATCAACAACTATTGCATTCATT |
mCRY-R | GCGGCCGCTTTACTTGTACAGCTCGTCCATGCC |
NeuroD-F | CAAGGTGGTACCTTGCTACTCCAAG |
NeuroD-R | GGAATAGTGAAACTGACGTGCCT |
Mash-F1 | TGCCAGGCTCTCCTGGGAATGG |
Mash-R1 | CTGGTTCGGATAGATACAAATAG |
In the RT-PCR experiments, olfactory marker protein transcripts were amplified by primers Eco OMP and OMP Xho, Adenylyl cyclase 3 by AC3F and AC3R, Gαolf by GolfF2 and GolfR2, cyclic nucleotide gated channel A2 subunit by CNGF2 and CNGR, NeuroD by NeuroD-F and NeuroD-R, Mash1 by Mash-F1 and Mash-R1, GAPDH by GAPDH-F and GAPDH-R, receptor M72 by M72-F and M72-R, I7 receptor by I7-F and I7-R, P2 receptor by P2-F and P2-R, MOR118 by OR118F and OR118R, and MOR182-5 by OR203F and OR203R.