Table 2.
Genes and primer sequences used for qPCR
| Gene | Sequence | Tm | Assay range cycles | Primer efficiency |
| EF1A (EEF1A1) | For: 5-cacacggctcacattgca Rev:5-cacgaacagcaaagcgacc |
86 | 12–30 | 98% |
| CD34 (HPCA1) | For: 5-tccagagacaaccttgaagc Rev: 5-cttcttaaactccgcacagc |
85 | 18–32 | 100% |
| CD133 (PROM1) | For: 5-ttgcggtaaaactggctaag Rev: 5-tgggcttgtcataacaggat |
81 | 20–36 | 100% |
| KDR (VEGFR2) | For: 5-gtggggattgacttcaactg Rev: 5-tgtgctgttcttcttggtca |
85 | 17–35 | 98% |
| CD144 (CDH5) | For: 5-ttcatgacgtgaacgacaac Rev: 5-tccaccacgatctcatacct |
89 | 17–35 | 84% |