Figure 3.

Inhibition of nuclear EGFR transport and phosphorylation of DNA-PK by src inhibitor PP2. Confluent A549 cells were treated with src inhibitor PP2 at a concentration of 100 nM for 1 h. Subsequently cells were irradiated with 4 Gy and nuclear proteins were isolated at the time points given. Proteins were separated by SDS-PAGE and after blotting protein amounts were quantified by help of specific antibodies. Expression of specific proteins was quantified by densitometry, normalized to lamin B1 and shown as fold-induction relative to untreated control. Experiments were performed three times; shown are representative results.