. Author manuscript; available in PMC: 2008 Sep 21.

Published in final edited form as: Biochemistry. 2006 Oct 3;45(39):11867–11878. doi: 10.1021/bi060930g

Table 1.

Kinetic parameters for WT and H103W SmNACE

Substrate^a	SmNACE variant	K_m (μM )	V_max (μmol/min/mg)	cyclization^b (%)
NAD⁺	WT^c	38.5 ± 6.8	13.2 ± 2.6	~ 0.02
	H103W	32.4 ± 5.4	13.3 ± 0.9	6.8
NGD⁺	WT^c	23.4 ± 1.1	4.8 ± 0.1	85.0
	H103W	47.7 ± 4.7	11.5 ± 0.4	90.0

All reactions were performed at 37 °C in 10 mM potassium phosphate buffer (pH 7.4) in the presence of purified recombinant WT and H103W SmNACE. The kinetic parameter values were calculated from a nonlinear regression fit to the Michaelis-Menten equation.

Formation of cADPR or cGDPR given as % of total reaction products

Data taken from (16).