Figure 4.

polαts13 cdc20 double mutant arrested with cdc phenotype. (A) FACS analysis of single mutant DBts13 (polαts13) and double mutants polαts13 cdc20 and polαts13 cdc25 in a hydroxyurea reciprocal shift experiment at 0, 2, and 4 h after shift to the restrictive temperature. The hydroxyurea reciprocal shift experiments were performed as described in MATERIALS AND METHODS. Double mutant polαts13 cdc25 at 25°C is moderately elongated, resulting in a >1C profile at 0 h. (B) Phenotypes of single and double mutants of polαts13 strains. Cells were stained with DAPI and calcofluor after shift to the restrictive temperature for 4 h. Bar, 5.8 μm.