Table 3.

Analysis of polαts13 in cdc mutant backgrounds

Strain	Flow cytometry profilea	Phenotype at 36°C	Abnormal nuclear morphology (%)b		Viability at 4 h (%)
			4 h	6 h
polαts13	Mid S	polαts13-like	26	35	14
polαts13 cdc10	G1	cdc-like	0	7	55
polαts13 cdc20	G1-S	cdc-like	0	2	100
polαts13 cdc22	G1-S	cdc-like	0	2	50
polαts13 cdc2-3w	Early S	small, cut	25	33	5
polαts13 cdc21	Early S	polαts13-like	25	35	6
polαts13 cdc18	Mid S	polαts13-like	25	33	7
polαts13 cdc19	Mid S	polαts13-like	16	17	8
polαts13 polδts03	Mid S	polαts13-like	28	35	10
polαts13 cdc17	Mid S	polαts13-like	17	31	12
polαts13 cdc25	G2	cdc-like	0	0	114

^aFlow cytometry profile was analyzed 4 h after shift to the restrictive temperature. 

^bPercent of abnormal nuclear morphology was determined 4 and 6 h after shift to the restrictive temperature.