Table 4.
Genetic interactions of polαts mutants with cell cycle response genes
| Allele | Growth at 25°C | Phenotype at 36°C
|
|
|---|---|---|---|
| Cell | Nuclear | ||
| polαts | ++a | Heterogeneousb | Aberrantb |
| polαts rad1Δ | Synthetic lethalc | ||
| polαts rad3Δ | Synthetic lethalc | ||
| polαts rad9Δ | Synthetic lethalc | ||
| polαts rad17Δ | Synthetic lethalc | ||
| polαts rad26Δ | Synthetic lethalc | ||
| polαts11 cds1Δ | Synthetic lethalc | ||
| polαts13 cds1Δ | Forms microcolonies at 22°C | Heterogeneousb | Aberrantb |
| polαts rqh1Δ | —d | ||
| polαts chk1Δ | ++ | Small | Cut |
a
++ represents growth.
b
After 6 h at 36°C, cells exhibit a mixture of elongated and small cell morphology with 40% of cells displaying a mixture of aberrant nuclear morphology.
c
Spores fail to germinate.
d
Spores either fail to germinate or form microcolonies with reduced growth rate.