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In vitro oligomerisation of FliIE211A ATPase (FliIEA). Purified variant FliI ATPase (0.5 μM) was incubated in cross-linking buffer (20 mM Hepes pH 8.0, 0.1 M NaCl, 0.1 mM ethylenediaminetetraacetic acid and 1 mM DTT) in the presence of E. coli liposomes, with (+) or without (−) 0.1 mM disuccinimidylglutamate). Aliquots were precipitated (10% trichloroacetic acid), subjected to electrophoresis through an SDS 4–10% acrylamide gradient gel and Coomassie stained.
