FIGURE 2.

Inhibition of transport of M449C/L466C by CuPh and Cd²⁺. A, HeLa cells expressing M449C/L466C or the indicated control mutants, all in the background of WT-GLT-1, were preincubated in NaCl-containing medium with 10 μm CuPh for 5 min at room temperature, washed twice with choline chloride-containing solution, and subsequently d-[³H]aspartate transport was assayed as described under “Experimental Procedures.” M449C co L466C represents HeLa cells cotransfected with each of these two constructs. The values shown represent the percentage of activity after treatment with 10 μm CuPh relative to that obtained after preincubation in the absence of CuPh. Values represent the mean ± S.E. of at least three separate experiments each done in triplicates. B, HeLa cells expressing the indicated mutants were washed once with choline chloride-containing solution and assayed for transport in the presence or absence of 500 μm cadmium chloride. Values shown are the percentage activity in the presence of 500 μm cadmium chloride relative to that in its absence. Values represent the mean ± S.E. of at least three separate experiments each done in triplicates.