TABLE 1.
Cell surface proteins identified by LC-MS/MS with abundance differences between HT-hi/diss and HT-lo/diss Percent coverage, UniProt accession numbers, and summarized known functions are as indicated. Four molecules (underlined) were selected to verify their abundance difference in biochemical assays and their potential role in intravasation.
| Coverage | Accession number | Protein identified | Function |
|---|---|---|---|
| Cell surface proteins enriched in HT-lo/diss | |||
| 35.3 | P05556 | Integrin β-1 precursor (splice isoform 1) | Extracellular matrix protein interactions |
| 29.9 | P30450 | HLA class I histocompatibility antigen, A-26 α chain | Antigen presentation |
| 27.1 | P30447 | HLA class I histocompatibility antigen, A-23 α chain | Antigen presentation |
| 26 | P30459 | HLA class I histocompatibility antigen, A-74 α chain | Antigen presentation |
| 19.4 | P84095 | Rho-related GTP-binding protein RhoG | Cell motility/cytoskeletal rearrangement |
| 17.9 | O14828 | Secretory carrier-associated membrane protein 3 | Recycling |
| 15.5 | P16035 | Metalloproteinase inhibitor 2 precursor (TIMP-2) | Inhibition of active MMPs; activation of proMMP-2 |
| 11.6 | P14923 | JUP protein | Junctional plaque formation |
| 10.2 | Q96S97 | Myeloid-associated differentiation marker | Hematopoetic differentiation |
| 7.2 | P36897 | Transforming growth factor-β receptor type I precursor | Signaling in response to transforming growth factor-β |
| 6.8 | P11234 | Ras-related protein Ral-B | Signal transduction |
| 5.7 | P55287 | Cadherin-11 precursor | Homophilic cell adhesion |
| 5.1 | P49757 | Numb protein homolog | Cell fate during development |
| 4.9 | Q92542 | Nicastrin precursor | γ-Secretase complex |
| 4.1 | P23469 | Receptor-type tyrosine-protein phosphatase ε | Dephosphorylation |
| 4 | Q86UN3 | Nogo receptor-like 3 | Neuronal plasticity |
| 3.4 | Q14114 | Low density lipoprotein receptor-related protein 8 | Endocytosis of reelin/apolipoprotein |
| 1.8 | Q13443 | ADAM 9 precursor | Proteolysis, cell-cell, and cell-matrix interactions |
| 1.6 | Q8WWQ8 | Stabilin 2 precursor | Hyaluronic acid endocytosis |
| 0.9 | P98161 | Polycystin 1 precursor | Ion channel regulation |
|
0.2
|
Q8WX17
|
Ovarian cancer-related tumor marker CA125
|
Mucin barrier maintenance, heterotypic cell adhesion
|
| Cell surface proteins enriched in HT-hi/diss | |||
| 32.6 | P32970 | Tumor necrosis factor ligand superfamily member 7 | Cytokine signaling (CD27 ligand) |
| 33.6 | P13592 | Neural cell adhesion molecule-1 (NCAM-1) | Cell adhesion; neurite outgrowth |
| 19.1 | Q9BY32 | Inosine triphosphate pyrophosphatase | Hydrolyze ITP and dITP, possible oncogene |
| 26.5 | Q99536 | Synaptic vesicle membrane protein VAT-1 homolog | Oxidoreductase |
| 15.8 | Q658P3 | TSAP6 protein | Iron homeostasis, cell cycle regulation |
| 13.5 | Q12891 | Hyaluronidase 2 precursor | Hyaluronic acid hydrolysis |
| 22.3 | P18206 | Vinculin isoform VCL | Cell Adhesion |
| 15.1 | P13726 | Tissue Factor (TF) | Initiation of coagulation cascade |
| 16.4 | Q14118 | Dystroglycan precursor | Linkage of cytoskeleton to ECM |
| 10.9 | Q9HD45 | Transmembrane 9 superfamily protein member 3 | Nonaspanin protein, potential role in transport |
| 9.7 | Q01650 | Large neutral amino acids transporter small subunit 1 | Amino acid transport/uptake |
| 10.1 | Q9BX67 | Junctional adhesion molecule 3 precursor (JAM-C) | Cell-cell adhesion; migration |
| 12.3 | P37173-1 | Transforming growth factor-β receptor type II precursor | Signaling in response to transforming growth factor-β |
| 12.9 | Q9NZ53-1 | Podocalyxin-like protein | Leukocyte membrane protein |
| 13.3 | Q96JA1-1 | Leucine-rich repeats and Ig-like domains protein 1 | Negative feedback regulation of receptor tyrosine kinase signaling |
| 7.6 | Q9Y6N7-1 | Roundabout homolog 1 precursor | Migration (axonal guidance) |
| 10.2 | P22413 | Ectonucleotide pyrophospatase/phosphodiesterase 1 | ATP hydrolysis |
| 10.7 | P10586 | Receptor-type tyrosine-protein phosphatase F precursor | Possible adhesion receptor, PTPase |
| 10.4 | Q9ULF5 | KIAA1265 protein | Zinc transport, associated with invasive breast cancer |
| 4.8 | P05067 | Amyloid β A4 protein precursor | Adhesion, migration, neuronal outgrowth, signaling |
| 5.5 | P27544 | LAG1 longevity assurance homolog 2 | Lipid synthesis |
| 6.1 | P08069 | Insulin-like growth factor I receptor precursor | Signaling in response to insulin |
| 4.4 | P28827 | Receptor-type tyrosine-protein phosphatase μ | Cell to cell adhesion, growth, signaling |
| 6.2 | P54760 | Receptor protein-tyrosine kinase variant (EphB4v1) | Possible role in differentiation/development |
| 4 | Q04721 | Neurogenic locus notch homolog protein 2 precursor | Transcriptional regulation in response to ligand binding |
| 5 | Q8N5L2 | AXL receptor tyrosine kinase, isoform 1 | Protein phosphorylation |