FIGURE 2.

Inhibition of cell polarization toward a CXCL8 gradient in the Zigmond chamber. Cells pretreated with different inhibitors for 30 min at 37 °C were induced to polarize toward a CXCL8 gradient in the Zigmond chamber for 15 min at 37 °C. The vertical line in each figure panel indicates the interface of CXCL8-containing medium (left) and RPMI medium alone (right). A higher magnification image of the boxed section was shown below each panel of images for a clearer view of cell polarization. The percentage of cells polarized along the direction of the gradient was quantified as described under “Experimental Procedures.” The scale bar is 20 μm. The direction of the CXCL8 gradient (0–25 ng/ml) is indicated by the arrow.