FIGURE 3.

Inhibition of TNF-α-induced chromatin events in SET7/9 knockdown THP-1 cells. Wild type (CON) or SET7/9 stable knockdown THP-1 cells (SI) were treated with or without TNF-α for the indicated time periods, and ChIP assays were performed with anti-p65, anti-SET7/9, anti-H3K4-monomethyation, anti-H3K4-trimethylation, or anti-p300 antibodies. ChIP-enriched samples were analyzed by regular PCR using MCP-1 (A) and TNF-α (B) promoter primers. Specific locations of MCP-1 promoter primers are shown in supplemental Fig. 1. Data are representative of three separate experiments. B, real time QPCRs with ChIP-enriched DNA using MCP-1 promoter primers showing significant changes and confirmation of data in A and C (^*, p < 0.005 SI + TNF-α for 1 h versus CON + TNF-α for 1 h, n = 3).