FIGURE 4.

SET7/9 does not affect the binding activity of p65 to oligonucleotides containing NF-κB consensus binding sites: EMSA using³²P-labeled oligonucleotide probes corresponding to NF-κB binding site sequence in the MCP-1 promoter (top) or generic NF-κB consensus binding site (bottom) with nuclear extract from control (CON) or SET7/9 knockdown (SI) cells with and without TNF-α treatment. Specificity of DNA-protein complexes was confirmed by competition with cold unlabeled DNA oligonucleotides (lanes 4 and 6). The presence of p65 was confirmed by supershifting with p65 antibodies (lanes 10 and 13).