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. 2008 Sep 26;283(39):26374–26382. doi: 10.1074/jbc.M803136200

FIGURE 1.

FIGURE 1.

Opening of hemichannels and release of PGE2 are correlated with the magnitude of FFSS applied. A, MLO-Y4 cells were subjected to FFSS at 0, 4, 8, and 16 dynes/cm2 for 30 min. Immediately after flow, LY dye uptake was performed, and images were captured for quantitation. B, the number of MLO-Y4 cells with LY dye uptake in the presence or absence of FFSS were counted and quantified using a 10× objective. 4, 8, and 16 dynes/cm2 versus 0 dynes/cm2: **, p < 0.01; ***, p < 0.001. C, Alexa 594 dye uptake was performed after 30 min of FFSS at 16 dynes/cm2, and the number of MLO-Y4 cells with Alexa 594 dye uptake were counted and quantified. 16 dynes/cm2 versus 0 dynes/cm2: ***, p < 0.001. D, MLO-Y4 cells were subjected to FFSS at 0, 4, 8, and 16 dynes/cm2 for 30 min, and the media were collected. The amount of PGE2 in media was measured using a PGE2 EIA kit. 4, 8, and 16 dynes/cm2 versus 0 dynes/cm2: **, p <0.01; ***, p < 0.001. E, MLO-Y4 cells were subjected to control with no flow, C, steady flow, FF(St), at 16 dynes/cm2 or pulsatile flow, FF(Pu), at 8 ± 8 dynes/cm2 for 30 min and LY dye uptake was measured. FF(St) and FF(Pu) versus C: ***, p < 0.001. All data are presented as mean ± S.E. and n = 3.