FIGURE 6.

Inhibition of PGE₂ release, but not intracellular levels of PGE₂, by Cx43(E2) antibody in response to FFSS. MLO-Y4 cells were treated with or without preimmune (Pre) or Cx43(E2) antibody in the absence (C) or presence of FFSS (FF) at 16 dynes/cm² for 30 min (A and B) or for 10, 30, and 60 min. The media and cells were collected. The amount of extracellular (A), and intracellular (B and C) PGE₂ was measured using a PGE₂ EIA kit. In A, extracellular PGE₂ in FF and FF+Pre versus FF+Cx43(E2), C, C+Cx43(E2) and C+Pre: ***, p < 0.001. In B, intracellular PGE₂ in FF, FF+Cx43(E2) and FF+Pre versus C, C+Cx43(E2) and C+Pre: ***, p < 0.001. All data are presented as mean ± S.E. and n = 3.