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. 2008 Sep 26;283(39):26349–26356. doi: 10.1074/jbc.M803491200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Characterization of HCT116 mitochondrial repair complex for LP-BER. A, repair of unlabeled THF-containing oligo3 duplex with [α-³²P]dTTP and two different amounts of mitochondrial APE1-FLAG or control FLAG immunocomplex. B, monitoring of LP-BER with APE1-FLAG immunocomplex with [α-³²P]dNTPs. M, marker (43 nt). control, no protein. Western analysis of mitochondrial APE1-FLAG immunocomplex (C). The absence of APE1 (D) and presence of PCNA (E) in FEN-1 immunocomplex isolated from mitochondrial and nuclear extracts, respectively. Lysate, 50 μg of mitochondrial or nuclear extracts.