Skip to main content
. 2008 Sep 26;283(39):26577–26590. doi: 10.1074/jbc.M800423200

FIGURE 1.

FIGURE 1.

PDGF-BB induces cyclin A expression and CDK2 activity in NFAT-dependent manner in VSMC. A, quiescent VSMC were treated with and without PDGF-BB (20 ng/ml) for the indicated times, and cell extracts were prepared. An equal amount of protein from control and each treatment was analyzed either for cyclin A and CDK2 levels by Western blotting or for CDK2 activity by immunocomplex kinase assay. B, quiescent VSMC were treated with and without PDGF-BB (20 ng/ml) in the presence and absence of CsA (10 μm) for the indicated times, and cell extracts were prepared and analyzed for cyclin A and CDK2 levels and/or activity as described in A. C, all the conditions were the same as in B except that cells were transduced with either Ad-GFP (control) or Ad-VIVIT with an m.o.i. of 80 and quiesced before treatment with and without PDGF-BB (20 ng/ml) and analyzed for cyclin A and CDK2 levels and/or activity. The bar graphs represent mean ± S.D. values of three independent experiments. *, p < 0.01 versus control or GFP; †, p < 0.01 versus PDGF-BB or GFP + PDGF-BB treatment alone.