Skip to main content
. 2008 Sep 26;283(39):26577–26590. doi: 10.1074/jbc.M800423200

FIGURE 3.

FIGURE 3.

PDGF-BB induces the expression of cyclin A mRNA in NFAT-dependent manner in VSMC. A, quiescent VSMC were treated with and without PDGF-BB (20 ng/ml) for the indicated times, and total cellular RNA was isolated. An equal amount of RNA was subjected to RT-PCR analysis of cyclin A and β-actin mRNA levels using their respective primers. B, quiescent VSMC were treated with and without PDGF-BB (20 ng/ml) in the presence and absence of CsA (10 μm) for the indicated times, and total cellular RNA was isolated and analyzed for cyclin A and β-actin mRNA levels as described in A. C, conditions were the same as in B except that cells were transduced with either Ad-GFP or Ad-VIVIT at an m.o.i. of 80 and quiesced before subjecting to PDGF-BB treatment and RT-PCR analysis. The bar graphs represent mean ± S.D. values of three independent experiments. *, p < 0.01 versus control or GFP; †, p < 0.01 versus PDGF-BB, GFP + PDGF-BB treatment alone.