FIGURE 5.

Blockade of NFAT activation suppresses balloon injury-induced cyclin A expression and CDK2 activity in the arteries. Soon after balloon injury, the rats received adenovirus expressing either GFP or VIVIT by infusion into the injured arteries. Three days after balloon injury, rats were sacrificed, and the injured right common carotid arteries and uninjured left common carotid arteries were dissected out, and either tissue extracts or sections were made. A, an equal amount of protein from uninjured and balloon-injured Ad-GFP or Ad-VIVIT-transduced arteries was analyzed for cyclin A andβ-tubulin expression and CDK2 activity by Western blotting and immunocomplex kinase assay, respectively, as described in Fig. 1 legend. The bar graph represents mean ± S.D. values of six animals in three groups. B, double immunofluorescence staining of balloon injured Ad-GFP or Ad-VIVIT transduced carotid artery sections for SMα-actin and cyclin A. ^*, p < 0.01 versus UI; †, p < 0.01 versus GFP-BI. UI, uninjured.