Skip to main content
. Author manuscript; available in PMC: 2008 Sep 22.
Published in final edited form as: Cancer Res. 2006 Jan 15;66(2):1181–1190. doi: 10.1158/0008-5472.CAN-04-2962

Figure 2.

Figure 2

Antigen microarrays on biochip. Sixty-five clones in quintuplicate were robotically arrayed on biochip (FAST slides). Binding of antigens first with serum IgG from ovarian cancer patient and normal healthy individual and next with Cy3-labeled T7 anti-capsid antibody and Cy5-labeled anti-human IgG was done as described in Materials and Methods. The arrays were scanned at 532 and 635 nm lasers in an Axon Laboratories 4100A scanner. A, microarrays processed with serum IgG from control individual PM0574. A small section of the entire biochip. Arrows, five replicates of a particular clone; the location of the five replicates has been designated as r1_c2, r5_c2, r9_c2, r13_c2, and r17_c2 (r, row; c, column). B, microarrays processed with serum IgG from ovarian cancer patient PM0175. Arrows, five replicates of the same clone as shown in (A).