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Heat shock-inducible p53 nuclear import is inhibited in ALTR12 cells. (A) A1–5 or ALTR12 cells were treated with heat shock (42°C for 20 minutes) or azetidine at the concentration indicated. After incubation at 37°C for 3 hours, cells were stained for p53 localization using PAb421 antibody. The photomicrographs shown represent a typical result. (B) The fraction of nuclear localized p53 in A was quantified. Graphs show the mean ± SE from three independent experiments.
