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. 2008 Oct;10(10):1073–1082. doi: 10.1593/neo.07739

Figure 1.

Figure 1

Real-time PCR of TGFβ target genes identified by DNA array. The change in gene expression of genes in RIE-Ras(12V) cells treated for 1 hour with 2 ng/ml TGFβ1 is shown by the results from the array experiments as well as by real-time PCR experiments. The average change in gene expression determined by the four independent array experiments is shown in light gray. Real-time PCR was used with a selected set of genes to verify the results of the array experiments. The ΔΔCt for the genes relative to GAPDH represents fold-change after TGFβ treatment, shown in black. GAPDH and cyclophilin are housekeeping genes, the expression of which did not change significantly with TGFβ1 treatment. The genes identified by the array experiments as having increased transcription in RIE-Ras (12V) cells after TGFβ1 treatment were confirmed by real-time PCR experiments.