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. 2008 Oct;10(10):1073–1082. doi: 10.1593/neo.07739

Figure 4.

Figure 4

Induction of CTGF by TGFβ in RIE and RIE-Ras(12V) cells. (A) Northern blots were prepared from RNA isolated from RIE and RIE-Ras(12V) cells treated with 2 ng/ml of TGFβ1 for the time intervals shown and probed for CTGF expression. Equivalent loading was confirmed by the signal intensity of IB15, a constitutively expressed gene. (B) Relative expression of CTGF in RIE and RIE-Ras (12V) cells shows markedly reduced basal expression in the Ras-transformed line. Equivalent loading is confirmed by expression of 1B15, a constitutively expressed RNA species. (C) Organized identically to Figure 3D, and the RNA samples were identical to those used in Figure 3D. Cells were treated with 10 µM SB203580 (p38 kinase inhibitor), 10 µM U0126 (a MEK inhibitor), 1 µM RafKI (BAY439006, a Raf-1 kinase inhibitor), and 20 µM LY294002 (phosphoinositol 3-kinase inhibitor) for 24 hours and then treated with 2 ng/ml TGFβ1 for 1 hour before isolation of RNA according to procedures described in the Materials and Methods section. Equivalent loading was confirmed by hybridization with a cDNA probe complementary to cyclophilin. The result shown is representative of three separate experiments.