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. 1999 Jul;10(7):2441–2459. doi: 10.1091/mbc.10.7.2441

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Copyright © 1999, The American Society for Cell Biology

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Coexpression of Gas3/PMP22 and RhoA-V14 in NIH3T3 cells. (A) AK and rhoA-V14 were coexpressed with gas3/PMP22 in NIH3T3 cells. After 24 h from microinjection cells were fixed and processed for immunofluorescence to detect Gas3/PMP22FLAG. Survival and morphological changes were scored as described in the text. Data represent arithmetic means ± SD for seven independent experiments (survival rate, p = 0.225; altered shape, p < 0.001). (B) Immunofluorescence analysis of NIH3T3 cells coexpressing gas3/PMP22 and AK or gas3/PMP22 and rhoA-V14 or gas3/PMP22. NIH3T3 cells 24 h after seeding were microinjected with pEXV-gas3/PMP22FLAG (100 ng/μl) and pEXV-AK (25 ng/μl) or with pEXV-gas3/PMP22FLAG (100 ng/μl) and pEXV-RhoA-V14 (25 ng/μl). After 24 h cells were fixed and processed for immunofluorescence to visualize Gas3/PMP22FLAG. Bar, 5 μm.