Figure 3.

Tg skin grafts elicited anti-BM IgG in Wt and MHC I -/- mice. a. Tg skin grafts placed on Wt recipients elicited IgG that bound epidermal BMs in human and Tg skin (arrows, top and middle left panels), but not the skin of a Wt mouse (bottom left panel). Development of anti-BM IgG was unrelated to the grafting procedure (right panels). b: Longitudinal studies showed that Tg skin grafts placed on Wt (solid circles) and MHC I -/- (solid squares) recipients elicited high (640 to 2560) and durable (lasting ≥ 270 days) titers of anti-BM IgG that consistently appeared in the circulation of recipients 14 to 18 days after grafting. MHC II -/- mice (solid triangles) grafted with Tg skin did not develop anti-BM IgG. n=4/group in this longitudinal study that was representative of the aggregate work.