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. Author manuscript; available in PMC: 2008 Sep 22.
Published in final edited form as: J Invest Dermatol. 2007 Jul 26;127(12):2807–2817. doi: 10.1038/sj.jid.5700970

Figure 5.

Figure 5

CD4+ T cells from Wt mice grafted with Tg skin demonstrated specific and dose-related proliferative responses to NC16A-GST in vitro. Ten days after receiving grafts of Tg skin, axillary and inguinal lymph nodes of Wt mice were harvested and processed to yield CD4+ T cells that were placed in culture and assessed for secondary proliferative responses to various concentrations of recombinant NC16A-GST (solid circles), recombinant GST alone (solid squares), or PBS (solid triangles). γ-irradiated splenocytes from naïve, syngeneic Wt mice were used as stimulator cells. CD4+ T cells from graft recipients proliferated specifically to NC16A-GST but not to GST alone or PBS. CD4+ T cells from Wt mice grafted with Wt skin did not show proliferative response to NC16A-GST, GST, or PBS (data not shown). n=10/group.