TABLE 2.
Results of biological enrichment of Mycoplasma species by use of ATCC cell cultures
| Species | Lowest concn (CFU/ml) or serial dilution (M. hyorhinis only) detectable by PCRa
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vero cells
|
MDCK (NBL-2) cells
|
MDBK (NBL-1) cells
|
R9ab cells
|
293 (HEK-293) cells
|
Hep-G2 cells
|
|||||||
| 3 dpi | 7 dpi | 3 dpi | 7 dpi | 3 dpi | 7 dpi | 3 dpi | 7 dpi | 3 dpi | 7 dpi | 3 dpi | 7 dpi | |
| A. laidlawii | 0.5 | 0.025 | 0.25 | 0.05 | 0.25 | 0.1 | 0.25 | 0.1 | 10 | 0.25 | 0.25 | 0.2 |
| M. arginini | 0.5 | 0.25 | 0.25 | 0.1 | 0.5 | 0.25 | 0.5 | 0.25 | ND | ND | 0.25 | 0.25 |
| M. hyorhinis | 10−4 | 10−5 | 10−4 | 10−6 | 10−3 | 10−6 | 10−3 | 10−3 | 10−3 | 10−4 | 10−4 | 10−5 |
| M. fermentans | ND | 0.25 | ND | 0.025 | ND | 0.25 | ND | ND | ND | 2.5 | ND | ND |
| M. salivarium | NG | 1.0 | 1.0 | 0.25 | 0.25 | 0.5 | NG | 0.5 | NG | NG | NG | NG |
| M. orale | 0.25 | 0.25 | 0.25 | 0.05 | NG | 0.1 | 1.0 | 0.05 | NG | 0.05 | 1.0 | 0.05 |
| M. hominis | 0.25 | 0.25 | 0.1 | 0.1 | 1.0 | 0.25 | 0.5 | 0.01 | NG | 0.5 | 0.1 | 0.1 |
| M. gallisepticum | 0.5 | 0.05 | 0.25 | 0.05 | 1.0 | 0.25 | NG | 0.5 | NG | 0.1 | NG | 0.1 |
| M gallinaceum | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 1.0 | 0.5 | 0.25 | 0.25 |
| M. bovis | 0.05 | 0.1 | 0.05 | 0.05 | 0.25 | 0.1 | 0.1 | 0.1 | NG | 0.05 | 0.1 | 0.05 |
| M. synoviae | NG | NG | 0.5 | 0.05 | NG | NG | NG | NG | NG | NG | NG | NG |
a
NG, no growth; ND, not done.