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. 2008 Jul 18;74(17):5556–5562. doi: 10.1128/AEM.01156-08

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Copyright © 2008, American Society for Microbiology

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FIG. 3. — Confirmation of the amyE knockout (A), prophage 3 deletion (B) and hisZ in-frame deletion (C) by PCR. The PCR products were analyzed by agarose gel electrophoresis. Lanes 1 and 14, DNA markers. (A) P14 and P17 were used as primers, and water (lane 2), 168 (lane 3), 168 [ΔamyE::(lox71-spc-lox66)] (lane 4), and BSPC1 (ΔamyE::lox72)(lane 5) were used as templates. (B) P18 and P21 were used as primers, and water (lane 6), 168 (lane 7), BSPC7 [ΔamyE::(lox71-spc-lox66)] (lane 8), and BSPC2 (Δprophage 3::lox72) (lane 9) were used as templates. (C) P21 and P25 were used as primers, and water (lane 10), 168 (lane 11), 168 [ΔhisZ::(lox71-zeo-lox66)] (lane 12), and BSPC3 (ΔhisZ::lox72) (lane 13) were used as templates.