TABLE 1.
Strains and plasmids used in this study
| B. subtilis strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| 168 | trpC2 | BGSC |
| BSPC1 | ΔamyE::lox72 | This work |
| BSPC2 | Δprophage 3::lox72 | This work |
| BSPC3 | ΔhisZ::lox72 | This work |
| BSPC4 | ΔamyE::(lox72-spc-lox72 cassette) | This work |
| BSPC5 | ΔamyE::(lox72-spc-lox71 cassette) | This work |
| BSPC6 | ΔamyE::(lox72-spc-lox66 cassette) | This work |
| BSPC7 | ΔamyE::(lox71-spc-lox66 cassette) | This work |
| BSPC8 | ΔamyE::lox72 Δprophage 3::lox72 ΔhisZ::lox72 | This work |
| BSPC9 | ΔamyE::(mpd expression cassette, lox72) | This work |
| BSPC10 | ΔamyE::(Pspac-cre spc lacI) | This work |
| BSPC11 | ΔamyE::(Pspac-cre spc lacI) ΔhisZ::(lox71-zeo-lox66 cassette) | This work |
| BSPC12 | ΔhisZ::(lox71-zeo-lox66 cassette) | This work |
| BSPC13 | ΔlacA::(Pspac-cre spc lacI) | This work |
| Plasmids | ||
| pMD18-T | Ampr; MCS | Takara |
| p7S6 | pMD18-T ligated with lox71-spc-lox66 cassette | This work |
| p2S6 | pMD18-T ligated with lox72-spc-lox66 cassette | This work |
| p2S1 | pMD18-T ligated with lox72-spc-lox71 cassette | This work |
| p2S2 | pMD18-T ligated with lox72-spc-lox72 cassette | This work |
| pPICZαA | Zeor; P. pastoris expression vector | Invitrogen |
| p7Z6 | pMD18-T containing lox71-zeo-lox66 cassette | This work |
| pTS | Emr Ampr; temperature sensitive in B. subtilis | This work |
| pTSC | pTS containing Pspac-cre expression cassette | This work |
| pDG148 | Ampr Kmr; B.subtilis-E. coli shuttle expression vector | 33 |
| pDGC | pDG148 containing cre fused to Pspac promoter | This work |
| pDG1730 | Ampr Spcr; B. subtilis amyE locus integration vector | 18 |
| pDGI | pDG1730 containing IPTG-inducible expression elements | This work |
| pDGIC | pDGI containing cre under control of Pspac | This work |
| pDGICZ | pDGIC, Sh ble | This work |
| pAX01 | Emr Ampr; B. subtilis lacA locus integration vector | 19 |
| pAXC | Ampr Spcr; pAX01 containing cre IPTG-inducible expression cassette | This work |
| pM7Z6M | pMD18T-simple ligated with PCR-fused fragment containing lox71-spac-lox66 cassette and homology-flanking amyE site | This work |
| pUBC19 | Ampr Kmr; B.subtilis-E. coli shuttle vector | 39 |
| pPhisZ | pUBC19 containing hisZ gene and its promoter region | This work |
| pP43NMK | Ampr Kmr; B. subtilis expression vector, mpd expression cassette | 39 |