Strains |
|
|
168 |
trpC2 |
BGSC |
BSPC1 |
ΔamyE::lox72
|
This work |
BSPC2 |
Δprophage 3::lox72
|
This work |
BSPC3 |
ΔhisZ::lox72
|
This work |
BSPC4 |
ΔamyE::(lox72-spc-lox72 cassette) |
This work |
BSPC5 |
ΔamyE::(lox72-spc-lox71 cassette) |
This work |
BSPC6 |
ΔamyE::(lox72-spc-lox66 cassette) |
This work |
BSPC7 |
ΔamyE::(lox71-spc-lox66 cassette) |
This work |
BSPC8 |
ΔamyE::lox72 Δprophage 3::lox72 ΔhisZ::lox72
|
This work |
BSPC9 |
ΔamyE::(mpd expression cassette, lox72) |
This work |
BSPC10 |
ΔamyE::(Pspac-cre spc lacI) |
This work |
BSPC11 |
ΔamyE::(Pspac-cre spc lacI) ΔhisZ::(lox71-zeo-lox66 cassette) |
This work |
BSPC12 |
ΔhisZ::(lox71-zeo-lox66 cassette) |
This work |
BSPC13 |
ΔlacA::(Pspac-cre spc lacI) |
This work |
Plasmids |
|
|
pMD18-T |
Ampr; MCS |
Takara |
p7S6 |
pMD18-T ligated with lox71-spc-lox66 cassette |
This work |
p2S6 |
pMD18-T ligated with lox72-spc-lox66 cassette |
This work |
p2S1 |
pMD18-T ligated with lox72-spc-lox71 cassette |
This work |
p2S2 |
pMD18-T ligated with lox72-spc-lox72 cassette |
This work |
pPICZαA |
Zeor; P. pastoris expression vector |
Invitrogen |
p7Z6 |
pMD18-T containing lox71-zeo-lox66 cassette |
This work |
pTS |
Emr Ampr; temperature sensitive in B. subtilis
|
This work |
pTSC |
pTS containing Pspac-cre expression cassette |
This work |
pDG148 |
Ampr Kmr; B.subtilis-E. coli shuttle expression vector |
33 |
pDGC |
pDG148 containing cre fused to Pspac promoter |
This work |
pDG1730 |
Ampr Spcr; B. subtilis amyE locus integration vector |
18 |
pDGI |
pDG1730 containing IPTG-inducible expression elements |
This work |
pDGIC |
pDGI containing cre under control of Pspac
|
This work |
pDGICZ |
pDGIC, Sh ble
|
This work |
pAX01 |
Emr Ampr; B. subtilis lacA locus integration vector |
19 |
pAXC |
Ampr Spcr; pAX01 containing cre IPTG-inducible expression cassette |
This work |
pM7Z6M |
pMD18T-simple ligated with PCR-fused fragment containing lox71-spac-lox66 cassette and homology-flanking amyE site |
This work |
pUBC19 |
Ampr Kmr; B.subtilis-E. coli shuttle vector |
39 |
pPhisZ |
pUBC19 containing hisZ gene and its promoter region |
This work |
pP43NMK |
Ampr Kmr; B. subtilis expression vector, mpd expression cassette |
39 |