TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide | Sequence (5′→3′) | Function |
|---|---|---|
| P0_lcpf (lanes 1-2) | 5′AGCCAGGCCGACATCATGGTC3′ | Degenerate PCR primers based on homologous sequences for |
| P0_lcpr (lanes 1-2) | 5′GCAGGACGAACTTACGGAGCGC3′ | amplification of a 500-bp region of lcp by PCR |
| P1_oxiBf (lanes 3-4) | 5′GCTCTCCGCGTGTCATGGAAG3′ | Degenerate PCR primers based on homologous sequences for |
| P1_oxiBr (lanes 3-4) | 5′TGCCGTTGCTTGTAGCTCAGG3′ | amplification of a 500-bp region of oxiB by PCR |
| P2_ oxiABf (lanes 9-10) | 5′CCCGATGCAGCAGGCATG3′ | Degenerate PCR primers based on homologous sequences for |
| P2_ oxiABr (lanes 9-10) | 5′CGCAGCGACAGATGTTGCG3′ | amplification of a 500-bp region of oxiB into oxiA by PCR |
| P3_oxiAf (lanes 5-6) | 5′CATCGTCATCATGCCGGACACC3′ | Degenerate PCR primers based on homologous sequences for |
| P3_oxiAr (lanes 5-6) | 5′GGACACCGTGTCCGCGAC3′ | amplification of a 160-bp region of oxiA by PCR |
| _lcp-oxiBf (lanes 7-8) | 5′GACTGGCCAAGCAGCCGG3′ | Degenerate PCR primers based on homologous sequences for |
| _lcp-oxiBr (lanes 7-8) | 5′CCAGTACGTATCCCAGGAAACGTC3′ | amplification of a 500-bp region of lcp into oxiB by PCR |
| A_P0_lcpf | 5′AGCCAGGCCGACATCATGGTC3′ | Degenerate PCR primers based on homologous sequences for |
| A_P1_oxiBr | 5′TGCCGTTGCTTGTAGCTCAGG3′ | amplification of a 778-bp region of lcp into oxiB by PCR |
| B_P1_oxiBf | 5′GCTCTCCGCGTGTCATGGAAG3′ | Degenerate PCR primers based on homologous sequences for |
| B_P3_oxiAr | 5′GGACACCGTGTCCGCGAC3′ | amplification of a 1.9-kb region of oxiB into oxiA by PCR |
| C_P2_ oxiABf | 5′CCCGATGCAGCAGGCATG3′ | Degenerate PCR primers based on homologous sequences for |
| C_P3_oxiAr | 5′GGACACCGTGTCCGCGAC3′ | amplification of a 1.8-kb region of oxiB into oxiA by PCR |
| D_lcp-oxiBf | 5′GACTGGCCAAGCAGCCGG3′ | Degenerate PCR primers based on homologous sequences for |
| D_P1_oxiBr | 5′TGCCGTTGCTTGTAGCTCAGG3′ | amplification of a 1.9-kb region of lcp into oxiB by PCR |
| E_P1_oxiBf | 5′GCTCTCCGCGTGTCATGGAAG3′ | Degenerate PCR primers based on homologous sequences for |
| E_P2_oxiABr | 5′CGCAGCGACAGATGTTGCG3′ | amplification of a 1.8-kb region of oxiB into oxiA by PCR |
| Lcp_primer extension | 5′CCGCCCCTAACGCCCCGACG-3′ | 5′ end labeled with the fluorescence dye IRD800 to determine the size of the primer extension product |