TABLE 1.
Average numbers of colonies recorded on different media seeded with two composite samples processed by ultrasonication/dilution pretreatment (method 1)
| Mediuma | No. of colonies (avg ± SD [104 CFU/g dry weight composite soil]) of:
|
||
|---|---|---|---|
| Nonactinomycete bacteria | Micromonospora-like actinomycetes | Other actinomycetes | |
| GYM | 93.7 ± 16.3 | 0 | 2.0 ± 0.6 |
| Gause inorganic agar | 66.7 ± 15.0 | 0 | 0.4 ± 0.3 |
| Glycerol asparagine agar | 1.0 ± 0.6 | 0 | 0 |
| Modified starch casein agar | 9.3 ± 2.1 | 0 | 2.3 ± 0.6 |
| Sauton's agar | 34.5 ± 3.2 | 0 | 15.0 ± 0.7 |
| DNB gellan gum | 21.6 ± 1.7 | 0 | 0.01 ± 0.003 |
| SEA | 17.3 ± 0.6 | 0.01 ± 0.003 | 3.0 ± 1.5 |
| SEA plus NB | 6.7 ± 0.3 | 0.03 ± 0.01 | 2.0 ± 0.01 |
| DNBA | 10.0 ± 4.6 | 0.01 ± 0.003 | 0.01 ± 0.003 |
| DNBA plus NB | 4.3 ± 1.5 | 0.02 ± 0.01 | 0.01 ± 0.003 |
| ISP 3 | 32.0 ± 5.8 | 0.5 ± 0.3 | 1.0 ± 0.9 |
| ISP 3 plus NB | 17.7 ± 3.7 | 0.8 ± 0.3 | 0.4 ± 0.1 |
| HVA | 26.5 ± 1.5 | 0.2 ± 0.1 | 2.5 ± 1.5 |
| HVA plus NB | 17.5 ± 2.1 | 0.4 ± 0.1 | 0.7 ± 0.6 |
| HVG | 33.0 ± 0.6 | 0.01 ± 0.003 | 0.8 ± 0.6 |
| HVG plus NB | 11.0 ± 0.5 | 0.2 ± 0.03 | 0.9 ± 0.2 |
| GA | 3.0 ± 1.5 | 0.01 ± 0.05 | 0.3 ± 0.1 |
| GA plus NB | 0.8 ± 0.5 | 0.01 ± 0.003 | 0 |
| CM | 3.0 ± 1.5 | 0.2 ± 0.1 | 0.5 ± 0.4 |
| CM plus NB | 2.3 ± 0.7 | 0.4 ± 0.1 | 0.2 ± 0.3 |
a
All media contained cycloheximide, nystatin, and nalidixic acid (each at 50 μg/ml). NB, novobiocin at a concentration of 25 μg/ml.