TABLE 2.
Transposon insertion mutant stains obtained
| Mutant strain(s) | ORF designation/location in HD100 genome | Predicted gene product | Top additional BLASTX hit (expect value) |
|---|---|---|---|
| JA-A1 | Between Bd3518 and Bd3519 | Unknown | None |
| JA-A8 | Bd2428 | Putative serine protease | Protease of Bacillus sp. strain PD498 (9e−30) |
| JA-A10 | Bd1580 | Hypothetical protein | None |
| JA-B5 | Bd3170 | Hypothetical protein | None |
| JA-B8 | Bd2093 | Hypothetical protein | Hypothetical SRU-0795 protein of Salinibacter spp. (2e−10) |
| JA-B9 | Between Bd0542 and Bd0543 | Putative CsrA regulatory protein | None |
| JA-C1 | Bd1131 | Putative amino acid aminotransferase | Branched-chain amino acid transferase of Clostridium spp. (3e−95) |
| JA-C4 | Between Bd3061 and Bd3062 | Unknown | None |
| JA-C5 | Bd3534 | Putative carboxy-terminal protease | Carboxy-terminal protease of Oceanospirillum spp. (8e−39) |
| JA-E1 | Bd2325 | Conserved hypothetical protein with a putative HD-GYP hydrolase domain | Conserved hypothetical signal transduction HD-GYP protein of “Candidatus” spp. (2e−36) |
| JA-E7 | Bd1915 | Putative protein involved in secretion through the outer membrane | Outer membrane efflux protein of Optutus spp. (5e−25) |
| JA-F1 | Between Bd2739 and Bd2740 | Unknown | None |
| JA-G1 | Bd2615 | Conserved hypothetical protein | Conserved hypothetical protein of “Candidatus” spp. (4e−72) |
| JA-G6 and SJ-G8 | Bd3489 | Hypothetical protein | None |
| JA-G9 | Bd2033 | Conserved hypothetical protein | GA21854 of Drosophila persimilis (6e−04) |
| SJ-VE4 | Bd3375 | Putative prolipoprotein diacylglycerol transferase | None |