TABLE 1.
Phylogenetic affiliations and frequencies of cloned bacterial 16S rRNA gene ampliconsa retrieved from RO samples from the full-scale RO water treatment plant
| Closest relative in GenBank (accession no.) | Similarity (%)b | % of clones in indicated clone libraryc
|
||||
|---|---|---|---|---|---|---|
| F | UF | CF | M | P | ||
| Sphingomonas sp. strain MTR-71 (DQ898300.1) | 95 | 16.4 | 17.7 | 7.9 | 2.7 | |
| Sphingomonas subterranea (AB025014.1) | 98 | 1.7 | 1.5 | 2.2 | 3.3 | 0.9 |
| Sphingomonas sp. strain ORS 1497 (AJ968701.1) | 96 | 1.5 | 1.1 | 0.7 | ||
| Sphingomonas sp. strain DB-1 (AY947554.1) | 98 | 1.5 | 2.2 | 0.7 | ||
| Sphingomonas sp. strain BAC151 (EU131005.1) | 97 | 5.6 | 1.5 | 1.1 | 9.2 | 2.7 |
| Sphingomonas sp. strain HI-K4 (DQ205308.1) | 98 | 3.0 | 1.3 | 0.9 | ||
| Sphingomonas sp. strain BAC13P (EU131003.1) | 97 | 1.1 | 0.7 | 0.9 | ||
| Sphingomonas oligophenolica (AB365794.1) | 98 | 0.7 | ||||
| Sphingomonas sp. strain HTCC500 (AY584571.1) | 98 | 0.7 | ||||
| Sphingomonas suberifaciens (D13737.1) | 97 | 0.7 | ||||
| Sphingomonas sp. strain P2 (AB091683.1) | 97 | 0.7 | ||||
| Other Alphaproteobacteria (AY029562.1, AB271055.1, AM411913.1, DQ414680.1, AM286550.1, X97691.1, DQ177493.1, AY921677.1, and EU050759.1) | 94-99 | 1.1 | 3.0 | 3.3 | 8.6 | 0.9 |
| Acidovorax spp. (AM262110.1, Y18617.1, EF540489.1, and AF235013.1) | 99 | 1.1 | 1.5 | 1.1 | 2.1 | 0.9 |
| Burkholderia spp. (AB232330.1, AY752954.1, AB212237.1, and DQ156083.1) | 99 | 78.3 | 1.5 | 1.1 | 1.4 | 41.2 |
| Janthinobacterium spp. (AF174648.1, EF422171.1, and AJ551147.1) | 98-99 | 5.1 | 14.9 | 14.4 | 1.4 | 6.3 |
| Nitrosomonas spp. (AB000700.1, AY123811.1, and AY123797.1) | 95-99 | 0.6 | 1.5 | 1.1 | 4.6 | |
| Other Betaproteobacteria (AB120966.1, AB195750.1, AF236004.1, AJ556799.1, DQ413154.1, EU130968.1, AM236310.1, AF351219.1, AJ575695.1, AF204252.1, AB265946.2, and AF078758.1) | 93-99 | 4.5 | 2.2 | 4.7 | 16.2 | |
| Pseudomonas spp. (AM157452.1 and DQ178233.1) | 97-99 | 1.1 | 20.9 | 15.5 | 2.0 | |
| Lysobacter spp. (AB161360.1 and AB249682.1) | 95-96 | 0.6 | 6.0 | 0.9 | ||
| Legionella spp. (X73406.1 and AM747393.1) | 93-97 | 0.6 | 1.1 | 1.3 | 2.7 | |
| Other Gammaproteobacteria (AM396494.1, EF191354.1, AJ583181.1, and AM229325.1) | 97-99 | 1.7 | 1.5 | 2.2 | ||
| Deltaproteobacteria (AY921696.1, AF418174.1, and U41561.1) | 98 | 0.7 | 1.8 | |||
| Flavobacterium spp. (AM230485.1, DQ628949.1, EF520552.1, and EF540472.1) | 97-100 | 2.2 | 1.5 | 3.3 | 2.7 | |
| Other CFBd (AY780553.1, AB074940.1, DQ640688.1, and AY910857.1) | 92-98 | 1.5 | 1.1 | 2.0 | 4.5 | |
| Clostridium spp. (AY360624.1, X75909.1, AJ506120.1, AB288643.1, and AY935674.1) | 92-99 | 10.5 | 13.3 | 2.0 | 4.5 | |
| Other Firmicutes spp. (EF033503.1, AM745263.1, and AY766466.1) | 91-97 | 0.6 | 1.5 | 3.3 | ||
| Actinobacteria spp. (AB271048.1, AY368456.1, and AM410685.1) | 98 | 3.9 | ||||
| Chlorobium phaeobacteroides (AM050128.1) | 98 | 0.9 | ||||
| Uncultured candidate division OP11 (AF047573.1) | 92 | 1.5 | 2.0 | |||
| Acidobacteria spp. (DQ513986.1, AY921727.1, and EF032752.1) | 96-97 | 6.0 | 7.8 | 3.3 | 0.9 | |
| Planctomycetales sp. (AY942960.1, AY500064.1, AB116499.1, DQ676396.1, AJ290177.1, EF221226.1, and X81950.1) | 91-98 | 14.5 | 4.5 | |||
| Verrucomicrobiae spp. (AB305640.1, AB288576.1 and AB288579.1) | 92-97 | 3.0 | 4.4 | 2.1 | 0.9 | |
| Uncultured bacteria (AY917428.1, EF220517.1, EF663458.1, EU273223.1, AB288666.1, EF506959.1, EF688335.1, AB290357.1, and DQ241389.1) | 84-98 | 10.8 | 2.7 | |||
a
Amplicons were approximately 1.45 kb in size.
b
Percentage of similarity between the cloned 16S rRNA gene and its closest relative in the NCBI database.
c
F clone library, 179 clones; UF clone library, 67 clones; CF clone library, 90 clones; M clone library, 152 clones; P clone library, 112 clones.
d
CFB, Cytophaga-Flexibacter-Bacteroides spp.