FIG. 4.

Succinate uptake analysis using C. glutamicum cell suspension. C. glutamicum cells were suspended at an OD₆₁₀ of 3 in Tris-HCl buffer with [1,4-¹³C]succinate. The C. glutamicum R wild-type strain (black squares), the dcsT-deficient strain (white triangles), and the dcsT-overexpressing strain (white circles) were used. Intracellular succinate was analyzed by GC-MS, and the time course of changes in m2 isotopomer originating from the labeled succinate is shown. Mean values from three independent experiments are shown with standard errors (error bars).