TABLE 2.
Genes and primer sequences
| Gene symbol | GenBank accession no. | Protein name | IDa | Primer sequences (5′→3′)b | Amplicon length (bp) | Exon(s)c | Functional class |
|---|---|---|---|---|---|---|---|
| ACTB | NM_001101 | β-Actin | 1 | CTGGAACGGTGAAGGTGACA (F) | 140 | 5 | Cytoskeletal structural protein |
| AAGGGACTTCCTGTAACAATGCA (R) | 5 | ||||||
| GAPDH | NM_002046 | Glyceraldehyde-3-phosphate dehydrogenase | 2065 | AAGGTCGGAGTCAACGGATTT (F) | 66 | 2/3 | Glycolysis and gluconeogenesis |
| ACCAGAGTTAAAAGCAGCCCTG (R) | 3 | ||||||
| HPRT1 | NM_000194 | Hypoxanthine phosphoribosyl-transferase 1 | 2989 | TGGTCAGGCAGTATAATCCAAAGA (F) | 100 | 6 | Purine synthesis in salvage pathway |
| TCAAATCCAACAAAGTCTGGCTTA (R) | 7/8 | ||||||
| RPLP0 | NM_001002 | Large ribosomal protein P0 | 2507 | CCTCATATCCGGGGGAATGTG (F) | 95 | 4 | Ribosomal protein |
| GCAGCAGCTGGCACCTTATTG (R) | 4/5 | ||||||
| PGK1 | NM_000291 | Phosphoglycerate kinase 1 | 2387 | AAGTGAAGCTCGGAAAGCTTCTAT (F) | 71 | 4 | Glycolysis |
| AGGGAAAAGATGCTTCTGGG (R) | 5 | ||||||
| TBP | NM_003194 | TATA box binding protein | 2630 | TGCACAGGAGCCAAGAGTGAA (F) | 132 | 5/6 | RNA polymerase II transcription factor |
| CACATCACAGCTCCCCACCA (R) | 6 | ||||||
| GUSB | NM_000181 | β-Glucuronidase | 2929 | CTCATTTGGAATTTTGCCGATT (F) | 81 | 11 | Lysosomal exoglycosidase |
| CCGAGTGAAGATCCCCTTTTTA (R) | 12 | ||||||
| B2M | NM_004048 | β2-Microglobulin | 1535 | TGACTTTGTCACAGCCCAAGATA (F) | 75 | 2 | Beta chain of major histocompatibility complex class I molecule |
| CGGCATCTTCAAACCTCCA (R) | 3/4 |
a
Identification number of primer sequence entry in RTPrimerDB.
b
F, forward; R, reverse.
c
Exon (or, for splice site-spanning primers, exons) in which primer binds.