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HPLC analysis of the 49-kDa OMP isolated from Salmonella serovar Typhimurium by preparative SDS-PAGE. The sample (300 μg) was applied to a C8 HPLC column, which was prerun with acetonitrile-water (30:70), and the bound proteins were eluted with the same solvent system. Two major peaks were observed at 2.070 min and 3.673 min. The two minor peaks eluted are insignificant, below the background level.
