FIG. 1.

Inhibition ELISA with variable BAT concentrations. Soluble toxins or toxoids were used to inhibit equine BAT binding to pure neurotoxin adsorbed to 96-well plates. (A) Inhibition by soluble 150-kDa neurotoxin occurred in the range of 2 to 20 nM neurotoxin. (B) Toxoid 3 inhibited BAT binding with similar effectiveness as the parent toxin. (C) A commercial toxoid derived from the pure neurotoxin required at least 10-fold higher concentrations of antigen to partially prevent BAT binding to the plate-bound neurotoxin. (D and E) The inhibition profile produced by the Hmg toxin complex (D) and its corresponding toxoid (E). Each data point is the average absorbance from four wells on a single ELISA plate. Error bars are one standard deviation. Abs, absorbance.