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Sandwich ELISA of toxins and toxoids. Biotinylated anti-BoNT/A IgG was used to detect toxin or toxoid applied to 96-well plates. Separate dilutions of BoNT/A (○), toxoid 4 (□), toxoid 3 (•), toxoid 2 (▪), and toxoid 0 (♦) were applied to 96-well plates that were coated with rabbit anti-BoNT/A IgG. Each symbol is the average of a minimum of three data points. Error bars represent one standard deviation unit. Abs, absorbance.
