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. 2008 Sep 15;22(18):2564–2577. doi: 10.1101/gad.1682208

Figure 2.

Figure 2.

βC1 reduces accumulation of miR165/166 but increases accumulation of PHB and PHV transcripts. (A) Phenotypes of 18-d-old βC1 transgenic plants treated with 25 μM β-estradiol. Treated XVE:βC1-Myc transgenic plants were like wild-type plants, whereas treated XVE:HA-βC1 and XVE:βC1 transgenic plants showed upward-curled leaves. Bars, 5 mm. (B) Expression of miR165/166, miR167 and miR173 in β-estradiol-treated or untreated seedlings of transgenic plants. Stained rRNAs and tRNAs bands were used as loading controls. (C) PHB, PHV, and Actin transcript levels in β-estradiol-treated or untreated seedlings of XVE:βC1-myc, XVE:HA-βC1, and XVE:βC1 transgenic plants. Stained rRNAs were used as a loading control. Expression levels of miRNAs and mRNAs were calculated using the program of Image Gauge version 3.12 (Fuji) and the values of β-estradiol-treated samples were normalized to untreated samples. (D) Levels of expression of indicated genes in shoot apices of XVE:βC1 plants. Data show expression levels in XVE:βC1 relative to wild-type levels. Levels of the ETT/ARF3, FIL, and YAB5 transcripts in shoot apices of 15-d-old plants were measured by real-time RT–PCR. XVE:βC1 and wild-type plants were grown on MS medium with 25μM β-estradiol. Each value was normalized by reference to the level of ACTIN2 transcripts. Error bars are indicated.