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. 2008 Sep 15;22(18):2564–2577. doi: 10.1101/gad.1682208

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Figure 6. — βC1 suppresses JA responses. RNA samples from Col-0, as1-1, XVE:HA-βC1/Col-0, and XVE:HA-βC1/as1-1 treated with 100 μM MeJA were analyzed by Northern blots. XVE:HA-βC1/Col-0 and XVE:HA-βC1/as1-1 lines were pretreated with 50 μM β-estradiol to induce high-level expression of βC1. Each lane contained 15 μg of total RNA. Stained rRNAs were used as loading controls. COI1 transcripts showed no change with 100 μM MeJA. JA-responsive genes were grouped into A, B, and C depending on the effects of βC1. In A, JA-responsive transcript levels were reduced in XVE:HA-βC1/Col-0 lines but largely restored in the XVE:HA-βC1/as1-1 line. In B, JA-responsive transcript levels were only weakly repressed in XVE:HA-βC1/Col-0 lines and slightly recovered or no change were detected in the XVE:HA-βC1/as1-1 line. In C, no or little change was seen with JA-responsive transcripts in XVE:HA-βC1/Col-0 and XVE:HA-βC1/as1-1 lines.