Figure 4.

Brefeldin A treatment. Cells were grown on glass coverslips and induced to comparable expression levels by the addition of cadmium chloride in the concentrations indicated in Figure 3A. After treatment with 5 μg/ml Brefeldin A for 1 h at 37°C, cells were fixed and permeabilized with methanol and processed for immunofluorescence by incubation with a monoclonal antibody against TGN38 (2F7.1) followed by FITC-conjugated anti-mouse secondary antibody. (A) Wild-type TGN38; (B) S331A; (C) S331D.