Table 1.
Comparison of protein expression levels in cells expressing TGN38, S331A, and S331D
| Cell type | Transfected construct | CdCl2 (μM) | DATA SET 1
|
DATA SET 2
|
||
|---|---|---|---|---|---|---|
| Total peak areaa | Deviationb | Total peak areaa | Deviationb | |||
| NRK | None | None | 1633 | 1.00 | 3697 | 1.04 |
| Cos-7 | Wild-type TGN38 | 1.0 | 1675 | 1.02 | 3748 | 1.05 |
| Cos-7 | S331A | 0.5 | 1680 | 1.03 | 3667 | 1.03 |
| Cos-7 | S331D | 0.5 | 1665 | 1.02 | 3560 | 1.00 |
For each data set, equal numbers of cells were stimulated with the indicated concentrations of cadmium chloride, lysed, immunoprecipitated, and immunoblotted as described in MATERIALS AND METHODS. Levels of protein in different lanes on the same immunoblot were compared by density profile analysis.
a
Total peak area is defined as the sum of the peak areas generated by mature and immature forms detected in each cell line.
b
Deviation was calculated by normalizing total peak areas within each data set to the lowest value in that set.