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. Author manuscript; available in PMC: 2008 Sep 22.
Published in final edited form as: Cell. 2008 Jan 25;132(2):299–310. doi: 10.1016/j.cell.2007.11.047

Figure 6. Expression of NFATc1 in mutant mouse models with altered hair follicle stem cell biology.

Figure 6

(A-D) K14 expression (immunolocalization and K14-GFP) and NFATc1 immunolocalization are shown for grafted skin from WT (A), heterozygous stabilized β-catenin (ΔN) (B) and BMPR1a conditional knockout (cKO) mice (C and D). (E) Real Time PCR of NFATc1 mRNA levels upon MK treatment with BMP4 and/or the BMP inhibitor, noggin. Data are mean ± SEM. N=3 individual experiments. (F) The activity of a 3.6-kb NFATc1 promoter construct following treatment of MKs with BMP4 and/or noggin or PMA (P) and ionomycin (I). Data are mean ± SEM. N=3 individual experiments. (G) NFAT reporter activity upon BMP4, noggin or P/I treatment of MKs. Data are mean ± SEM. N=3 individual experiments. Dapi staining (blue) shows nuclear localization. Bu, bulge, CN, calcineurin, DP, dermal papillae, HG, hair germ, epi, epidermis.