TABLE 3.

Inter- and intra-assay reproducibility for the triplex PyV assay

PyV	Log no. of genomes	Interassay variability				Intra-assay variability
		Avg Cp	SD	% CV	na	Avg Cp	SD	% CV	nb
JCPyV	1	37.02	1.17	3.15	6	37.98	1.35	3.54	24
	4	28.57	0.46	1.62	6	28.29	0.33	1.16	20
	5	25.30	0.34	1.34	6	24.59	0.46	1.88	19
	6	21.56	0.24	1.10	6	21.37	0.12	0.58	20
BKPyV	2	36.14	0.64	1.77	6	36.84	0.61	1.66	24
	4	29.96	0.30	1.00	6	30.01	0.41	1.38	20
	5	25.76	0.47	1.84	6	26.35	0.55	2.09	19
	6	23.07	0.49	2.13	6	23.01	0.10	0.43	20
MuPyV	2c	35.09	0.22	0.64	6	35.10	0.22	0.64	92
	2d	36.73	0.70	1.91	18	37.11	1.03	2.77	72

^an, Number of replicate qPCR runs analyzed.

^bn, Number of individual replicates within one qPCR run analyzed.

^cMuPyV plasmid DNA was added to qPCR master mix prior to amplification.

^dMuPyV plasmid DNA was added to clinical samples prior to DNA extraction.