Sequence alignment of a 233-nucleotide fragment of lpnA of representative subspecies of F. tularensis and FLE, as well as F. philomiragia (nucleotide positions 593 to 825, as in F. tularensis subsp. holarctica strain LVS [GenBank accession no. M32059]). Boldfaced nucleotides represent primers, and underlined nucleotides represent probes, as in Table 1. The accession numbers are as follows: F. tularensis subsp. holarctica-1 strain LVS, GenBank no. M32059; F. tularensis subsp. holarctica-2 strain LVS, GenBank no. AM261157; F. tularensis subsp. tularensis strain Schu S4, AJ749949; F. tularensis subsp. novicida strain Utah 112, CP000439; unusual F. tularensis strain FnSp1, EU363482; F. tularensis novicida-like strain, AY243029; FLE of Dermacentor variabilis, AY375420; FLE of D. variabilis, AY375421; FLE of Dermacentor andersonii, AY375413; FLE of Amblyomma maculatum, AY375422; FLE of Dermacentor hunteri, AY375417; F. philomiragia strain 2669, AY243030.