TABLE 1.
Characteristics of the MLVA scheme
| Locus (RU size [bp]) | Formulaa | No. of RUs (% PCR negative) |
|---|---|---|
| SIRU01 (55) | (n − 157 − 30)/55 | 0-5 (4.5) |
| SIRU05 (60) | (n − 76 − 78)/60 | 1-22 (21.5) |
| SIRU07 (56) | (n − 27 − 160)/56 | 1-4 (2.2) |
| SIRU13 (64) | (n − 76 − 78)/64 | 0-26 (2.2) |
| SIRU15 (131) | (n − 48 − 174)/131 | 0-5 (0) |
| SIRU21 (24) | [(n − 12 − 81) − 16)]/24 | 1-16 (0) |
a
The formula for calculating the number of RUs per locus is as follows: (amplicon size [n] − size of left flanking region − size of right flanking region)/size of RU.