FIG. 2.

Phylogenetic tree based on 16S rRNA gene sequence comparison over 541 aligned bases between the Treponema phylotypes isolated from DD lesions in this (marked in bold) and previous studies and cultivable strains of Treponema. Borrelia burgdorferi was included as an outgroup. FISH probe numbers (in parentheses) and GenBank accession numbers are shown. The tree was constructed by the neighbor-joining method, and the similarity matrices were corrected for base changes using the Jukes and Cantor correction. The scale bar represents a 5% difference in nucleotide sequences.