FIG. 4.
Bias between targeted IRs. In vitro competition insertion experiments were performed using two target plasmids. The symbols are the same that those presented in Fig. 3. Ori (from pBR plasmids) and the ampicillin resistance gene (bla) are indicated. Mutated IRs for the 5′-CA are represented with *. The substrate carries an IRL-IRR junction and a Cmr gene represented in the bold circle. Three OrfAB concentrations (0.32, 0.75, and 1.26 μM) were used for the reactions. The numbers of IRR*-targeted and IRL*-targeted events are indicated as the ratio between the two events.