FIG. 6.

Bias between donor IRs. The symbols are the same that those presented in previous figures. The three transposon circles used as substrates are shown. They contain either the IRL-IRR wild-type junction, the IRL*-IRR mutated junction, or the IRL-IRR* mutated junction. The target plasmid is pBST1, and it carries a mutated IRL end (IRL*), an orfA-lacZ gene fusion, a pBR322 origin of replication (black oval), and the ampicillin resistance gene (bla). The OrfAB and OrfA concentrations were, respectively, 0.85 μM and 3.2 μM. The number of total insertion events measured is indicated as the total.